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radicals with sub-micron palmitic acid (PA) particles was studied in an aerosol flow tube in the presence or in the absence of O2. Reactivity of chlorine radical with submicron palmitic acid particles: kinetic measurements and products identification One particular application is the ordered transport and identification of labeled nucleotides sequentially cleaved from a strand of DNA. The time that the particles pass selected locations along the transport tube may then be detected and the electrophoretic velocity component calculated for particle identification. When the particles also have known electrophoretic velocities, the field gradients introduce an electrophoretic velocity component onto the electroosmotic velocity.
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Thus, particles are maintained in the same order in which they are introduced in the transport tube. The transport tube and electrolytic solution are selected to provide an electroosmotic radius of >100 so that a plug flow profile is obtained for the electrolytic solution in the transport tube. Particle sequence is maintained by providing electroosmotic flow for an electrolytic solution in a particle transport tube. Ordered transport and identification of particlesĪ method and apparatus are provided for application of electrical field gradients to induce particle velocities to enable particle sequence and identification information to be obtained. and the American Pharmacists Association. The techniques and observations described will be useful for the correct identification of proteinaceous versus nonproteinaceous particles in pharmaceutical products. In addition, particles containing a mixture of protein and fatty acids were also identified, suggesting that the particulation pathways for the two particle types may not be distinct.
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The particles were readily distinguishable from protein particles by these methods. They could be identified by distinct IR bands, with additional confirmation from energy-dispersive X-ray spectroscopy analysis. The fatty acid particles were granular or sand-like in morphology and were several microns in size. These fatty acid particles were observed in multiple mAb formulations during their expected shelf life under recommended storage conditions. We report, for the first time, the identification of fatty acid particles in formulations containing the surfactant polysorbate 20. Free fatty acid particles in protein formulations, part 1: microspectroscopic identification.Ĭao, Xiaolin Fesinmeyer, R Matthew Pierini, Christopher J Siska, Christine C Litowski, Jennifer R Brych, Stephen Wen, Zai-Qing Kleemann, Gerd R
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